The objective of the study is to assess the impact of genetic variation (especially polymorphisms of the gene coding endothelial nitric oxide synthase (eNOS) and the bradykinin B2 receptor gene) on venous and arterial responsiveness to vasodilators in healthy individuals without cardiovascular risk factors.

NCT00160888

Conditions

1. Healthy

Interventions

1. Drug: sodium nitroprusside, bradykinin, acetylcholine

Endothelium-Dependent Vasoreactivity in Nitric Oxide Synthase Gene (Glu298Asp) Polymorphism: Studies in Healthy Volunteers. --- Glu298Asp ---

Experimental elevation of non-esterified fatty acids (NEFA) impairs endothelial function and insulin sensitivity but the impact of NEFA composition is unknown. The objective was to test the effect of acute elevation of NEFA enriched with either saturated fatty acids (SFA) or SFA with long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) on postprandial vascular function measured via flow-mediated dilatation (FMD), laser Doppler iontophoresis (LDI) and digital volume pulse (DVP), followed by a hyperinsulinaemic-euglycaemic clamp as a measure of whole body insulin sensitivity.

NCT01351324

Conditions

1. Cardiovascular Risk Factors

Interventions

1. Dietary Supplement: Absence or presence of fish oil

MeSH:Insulin Resistance

HPO:Insulin resistance

Inclusion Criteria: - Healthy - Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant) Exclusion Criteria: - Smokers - Raised fasting blood lipids - Taking excessive fish oil supplements (>1g EPA/DHA per day) - Taking medication known to influence blood lipids, blood pressure or blood clotting Inclusion Criteria: - Healthy - Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant) Exclusion Criteria: - Smokers - Raised fasting blood lipids - Taking excessive fish oil supplements (>1g EPA/DHA per day) - Taking medication known to influence blood lipids, blood pressure or blood clotting Cardiovascular Risk Factors Insulin Resistance To investigate potential diet-gene interactions, potential subjects (n=370) were prospectively genotyped for the eNOS Glu298Asp polymorphism, of which 35 were Asp298 and 150 were Glu298 homozygotes. --- Glu298Asp ---

The Glu298Asp polymorphism has been linked to increased risk of cardiovascular events, proposed to be due to reduced nitric oxide production by eNOS reported in some but not all studies. We hypothesise that beetroot-enriched bread interacts with the Glu298Asp polymorphism of the eNOS gene to lower blood pressure and increase plasma nitrate/nitrite, particularly in carriers of the T allele, which have been associated with lower eNOS activity and increased risk of hypertension.

NCT02037308

Conditions

1. Hypertension

Interventions

1. Dietary Supplement: Bread

MeSH:Hypertension

HPO:Hypertension

The Effects of Beetroot Bread on Postprandial Blood Pressure According to Glu298Asp Polymorphism in the eNOS Gene. --- Glu298Asp ---

Glu298Asp Polymorphism and Blood Pressure The Glu298Asp polymorphism has been linked to increased risk of cardiovascular events, proposed to be due to reduced nitric oxide production by eNOS reported in some but not all studies. --- Glu298Asp ---

Glu298Asp Polymorphism and Blood Pressure The Glu298Asp polymorphism has been linked to increased risk of cardiovascular events, proposed to be due to reduced nitric oxide production by eNOS reported in some but not all studies. --- Glu298Asp --- --- Glu298Asp ---

We hypothesise that beetroot-enriched bread interacts with the Glu298Asp polymorphism of the eNOS gene to lower blood pressure and increase plasma nitrate/nitrite, particularly in carriers of the T allele, which have been associated with lower eNOS activity and increased risk of hypertension. --- Glu298Asp ---