SNPMiner Trials (Home Page)
Report for SNP rs679620
Developed by Shray Alag, 2020.
SNP Clinical Trial Gene
There is one clinical trial.
Clinical Trials
1 Design of a Protocol for Obtaining Genomic DNA From Saliva Using Mouthwash: Samples Taken From Patients With Periodontal Disease
Objective The aim of this study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease. Materials and methods Saliva samples were taken from 60 patients, then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.
NCT02523326 Periodontal Disease Periodontal Disease " Periodontal Disease "Unrecognized Condition Periodontal Disease "Unrecognized Condition" Device: saliva samples obtained with mouthwash MeSH:Periodontal Diseases
HPO:Periodontitis
To verify the quality and species (human) of the DNA extracted, primers were designed for the rs679620 polymorphism (forward 5´- ggg gCT TAA ggC ACA TgA gT-3; reverse 5´- ACT TCg ggA TgC CAg gAA Ag - 3´; Tm: 56°C, 40 cycles) that amplified a product of 485 bp.
Primary Outcomes
Description: high quality genomic DNA from saliva samples obtained with mouthwash.
Measure: DNA Time: 10 days [group A], 20 days [group B], 30 days [group C]