SNPMiner Trials (Home Page)
Report for Mutation G238A
Developed by Shray Alag, 2020.
SNP Clinical Trial Gene
There is one clinical trial.
Clinical Trials
1 Case Control Study of the Risk Factors for Pressure Ulcers in Tunisian Patients
Development of pressure ulcer (PU) is complex and multifactorial. The association of a constituted PU and of clinical / biological major elements is demonstrated and justifies. Prevention of PU is an important health priority, one that requires clear identification of risk factors.
NCT02578004 Pressure Ulcers Other: biochemical and molecular analysis MeSH:Pressure Ulcer Ulcer
HPO:Pressure ulcer
The relationship between the polymorphism of the MMP-9 gene and the severity of PU was analyzed.. Genotyping of TNF- α G238A. --- G238A ---
TNF-α G238A promoter polymorphism were determined by the RFLP-PCR method. --- G238A ---
Primary Outcomes
Description: Body Mass Index (BMI) is a simple index of weight-for-height. It is defined as the weight in kilograms divided by the square of the height in metres (kg/m2).
Measure: Anthropometric characteristics Time: one hourDescription: - Plasma levels glucose in mmol/l was measured by standard enzymatic methods using reagents in a fully automated analyzer Cx5 Pro-Bechman Coulter-Fuller-Ton
Measure: Diabetes mellitus Time: one hourDescription: Lipid markers: total serum cholesterol (CH), serum triglyceride, serum HDL- CH, in mmol/l, levels were measured by standard enzymatic methods using reagents in a fully automated analyzer (Randox Antrim, UK; CX9-BECKMANN). Low density lipoprotein cholesterol (LDL-C) in mmol / l was determined by Friedewald formula. non esterified fatty acids in serum was determined by colorimetric method at 550 nm (mmol/l)
Measure: Dyslipidemia Time: one hourDescription: - renal profile: urea (mmol/l), creatinine and uric acid (μmol/l) levels were measured by standard enzymatic methods using reagents in a fully automated analyzer ( Cx9 Pro-Bechman Coulter-Fuller-Ton).
Measure: Renal failure Time: one hourDescription: - C-reactive protein (CRP), in mg/l, was measured using immunoturbidimetric methods (COBAS INTEGRA 400 Roche).
Measure: Inflammatory parameter Time: one hourDescription: - α1‐acid glycoprotein, in g/l, measured using the dry chemistry method (BN prospec, siemens)
Measure: Endogenous inflammatory marker Time: one hourDescription: albumin (chronic marker) and prealbumin (early marker) were measured, in g/l, using the dry chemistry method (BN prospec, siemens). Protide in g/l was measured by standard enzymatic methods using reagents in a fully automated analyzer (CX9-BECKMANN).
Measure: Markers of nutritional status Time: one hourDescription: Serum total homocysteine concentrations in μmol/l were measured by using an AxSYM (ABBOTT) homocysteine assay. thiobarbituric acid reactive substances (TBARS) in serum was determined by the fluorimetric method of Yagi in μmol/l.
Measure: Marker of lipid peroxidation Time: one dayDescription: - Serum catalase activity in KU/l was determined according to the spectrophotometric method of Goth .
Measure: Antioxidant parameters Time: one dayDescription: Serum total antioxidant status in mmol/l was measured with RANDOX kit (Cat. No. NZ 2332; Randox Labs Ltd., Crumlin, UK) by colorimetric method at 600 nm .
Measure: Total antioxidant status Time: one hourDescription: Serum copper in μmol/l was indicated spectrophotometrically with RANDOX kit (Cat. No. CU 2340; Randox Labs Ltd., Crumlin, UK) at 580 nm according. Serum zinc was measured in μmol/l with RANDOX kit (Cat. No. ZN 2341; Randox Labs Ltd, Crumlin, UK) at 560 nm.
Measure: Determination of trace elements Time: one hourDescription: - Nutritional Risk Index (NRI) was originally derived from the serum albumin concentration and the ratio of present to usual weight [NRI = (1.489 x albumin, g/L) + (41.7 x present weight/ideal body weight)] and categorized as follows: severe risk (NRI < 83.5), moderate risk (83.5 < NRI < 97.5) and no risk (NRI > 97.5).
Measure: Nutritional status Time: 3 hoursDescription: - Prognostic Inflammatory and Nutritional Index (PINI) is a simple clinical [PINI = AAG x CRP / albumine x prealbumin] and classificated as follows: normal (1
Description: The bacterial colonization of a wound is a recognized detrimental factor in the multifactorial process of wound healing. wound per patient suffering from pressure ulcer was cultured by swab to determine the bacterial species of the infection and helps guide antibiotic therapy. The representative sample is collected before topical or systemic antibiotics are initiated and pain assessment should be conducted prior to wound procedures such as dressing changes and debridement. Bacterial swabs provide information on the predominant flora.
Measure: A microbiological diagnosis Time: 3 daysDescription: - Serum gelatinase activities of MMP-9 by zymography (%)
Measure: Proteomics Time: 2 daysDescription: Genomic DNA was extracted from whole blood using the salting out method for the part of molecular biology.
Measure: DNA extraction Time: 2 daysDescription: Genetic polymorphism in the MMP9 coding region 1562C>T was screened following the polymerase chain reaction and restriction fragment length polymorphism (RFLP-PCR). The frequency distributions of different MMP9-1562 C/T genotypes and allele were investigated. The relationship between the polymorphism of the MMP-9 gene and the severity of PU was analyzed.
Measure: Genotype for the MMP9-1562 C/T polymorphism Time: 1 daysDescription: TNF-α G238A promoter polymorphism were determined by the RFLP-PCR method. The genotypic and allelic frequencies of -238G/A were calculated This study investigated the association between TNF-α−238G>A and Pressure ulcer in Tunisian population.
Measure: Genotyping of TNF- α G238A Time: 1 daysDescription: The genotypic analysis of the TNF-α G308A polymorphism was performed using Allele-specific PCR (AS-PCR) amplification. In this study, we have analyzed the TNF-α gene promoter -308G/A polymorphism in Tunisian patients with PU to evaluate the contribution of this SNP in genetic susceptibility to PU.
Measure: Genotyping of TNF- α G308A Time: 1 days