Objective The aim of this study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease. Materials and methods Saliva samples were taken from 60 patients, then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.

NCT02523326

Conditions

1. Periodontal Disease
2. Periodontal Disease "
3. Periodontal Disease "Unrecognized Condition
4. Periodontal Disease "Unrecognized Condition"

Interventions

1. Device: saliva samples obtained with mouthwash

MeSH:Periodontal Diseases

HPO:Periodontitis

To verify the quality and species (human) of the DNA extracted, primers were designed for the rs679620 polymorphism (forward 5´- ggg gCT TAA ggC ACA TgA gT-3; reverse 5´- ACT TCg ggA TgC CAg gAA Ag - 3´; Tm: 56°C, 40 cycles) that amplified a product of 485 bp.