EBI2 is a risk gene for inflammatory bowel diseases (rs9557195). Patients of the Swiss IBD cohort study have been genotyped for the allelic status of EBI2. The investigators will test the influence of rs9557195 genoty (TT or CC allel), inflammatory activity and current treatment (infliximab vs. vedolizumab) on expression and activity of EBI2 on blood lymphocytes, mRNA expression of EBI2 and UBAC2 (located on the opposite DNA strand of EBI2) and activity of lymphocytes on a migraton assay.

NCT03633409

Conditions

1. Inflammatory Bowel Diseases

MeSH:Inflammatory Bowel Diseases

HPO:Inflammation of the large intestine

Differential EBI2 Expression Upon Allelic Status of the rs9557195 Polymorphism.

Differential EBI2 Expression Upon Allelic Status of the rs9557195 Polymorphism EBI2 is a risk gene for inflammatory bowel diseases (rs9557195).

The investigators will test the influence of rs9557195 genoty (TT or CC allel), inflammatory activity and current treatment (infliximab vs. vedolizumab) on expression and activity of EBI2 on blood lymphocytes, mRNA expression of EBI2 and UBAC2 (located on the opposite DNA strand of EBI2) and activity of lymphocytes on a migraton assay.

EBI2 expression - dependent on rs9557195 allele status (FACS).

EBI2 levels in PBMCs will be determined by FACS, and compared according to allele status of rs9557195.

EBI2 expression - dependent on rs9557195 allele status (RT-PCR).

EBI2 levels in PBMCs will be determined by RT-PCR, and compared according to EBI2 levels in PBMCs will be determined by RT-PCR, and compared according to allele status of rs9557195.

UBAC2 expression - dependent on rs9557195 allele status (RT-PCR).

UBAC2 levels in PBMCs will be determined by RT-PCR, and compared according to 2 levels in PBMCs will be determined by RT-PCR, and compared according to allele status of rs9557195.

Motility of PBMCs dependent on rs9557195 allele status (Boyden chamber).

motility of PBMCs will be assessed using the Boyden chamber and compared according to allele status of rs9557195.

medically controlled arterial hypertension, occupational asthma) may be present Exclusion Criteria: none Inflammatory Bowel Diseases Inflammatory Bowel Diseases EBI2 is a risk gene for inflammatory bowel diseases (rs9557195).

The investigators will test the influence of rs9557195 genotype (TT or CC allel), inflammatory activity and current treatment (infliximab vs. vedolizumab) on expression and activity of EBI2 on blood lymphocytes (by FACS using an EBI2-antibody), mRNA expression (by qPCR) of EBI2 and UBAC2 (located on the opposite DNA strand of EBI2) and activity of lymphocytes on a migration assay.