SNPMiner Trials (Home Page)
Report for Mutation I191V
Developed by Shray Alag, 2020.
SNP Clinical Trial Gene
There is one clinical trial.
Clinical Trials
1 Effect of Fructose and Glucose and TAS1R2 in Glucose, Triglycerides, Uremia, Oxidative Stress, Feelings Related to Food Intake of Individuals With Type 1 Diabetes
- BACKGROUND: Individuals with diabetes report innate preference for sweet foods, possibly due to genetic variants. In addition, studies have shown that ingestion of fructose promotes lower postprandial blood glucose, compared to glucose. However, excessive intake may increase triglycerides, uric acid and oxidative stress, due to oxidative priority. - AIMS: To investigate the influence of fructose and glucose and taste receptor, type 1, member 2 (TAS1R2) in glucose, triglycerides, uremia, oxidative stress, feelings related to food intake and palatability of individuals with type 1 diabetes. - METHODS: The trial is a single-blind, two-way crossover (1-week washout) study in 30 subjects with type 1 diabetes. Blood samples were collected before and 2-hours after the participants receive 75g of fructose or 75g of glucose dissolved in 200 ml water. Capillary blood glucose were assessed at 30, 90, 120, and 180 minutes to determine glucose, and visual analogue scales for measurement of appetite sensation were assessed at 70, 120, and 190 minutes. - PURPOSE: The research proposal adds knowledge about the TAS1R2 (Ile191Val) polymorphism and around the most suitable monosaccharide for individuals with diabetes type 1.
NCT01713023 Type 1 Diabetes Dietary Supplement: Glucose Dietary Supplement: Fructose MeSH:Diabetes Mellitus Diabetes Mellitus, Type 1
HPO:Diabetes mellitus Type I diabetes mellitus
- PURPOSE: The research proposal adds knowledge about the TAS1R2 (Ile191Val) polymorphism and around the most suitable monosaccharide for individuals with diabetes type 1. Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes. --- Ile191Val ---
We will determine whether Ser9Cys (dbSNP: rs9701796) and Ile191Val (dbSNP: rs35874116)variations in TAS1R2 were associated with differences the sweet taste perceptions.. Effect of fructose or glucose on blood glucose. --- Ser9Cys --- --- Ile191Val ---
The Ile191Val (rs35874116) polymorphism in TAS1R2 gene will be detected by using a TaqMan allelic discrimination assay (ABI number C_55646_20; Applied Biosystems, Foster City, CA) with real-time polymerase chain reaction on an ABI 7000 Sequence Detection System (Applied Biosystems). --- Ile191Val ---
Primary Outcomes
Description: Determine the blood glucose, triglycerides, oxidation of substrates, uremia, oxidative stress, feelings related to food intake of individuals with type 1 diabetes, before and 3-hours after ingestion of 75g of fructose or 75g of glucose.
Measure: Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes Time: baseline up to 3 hours each day study. Total study: 2 yearsSecondary Outcomes
Description: Determine the peak plasma blood glucose before and 3-hours after ingestion of 75g of fructose or 75g of glucose. We also test capillary blood glucose before and 30, 90, 120, and 180 minutes after solutions.
Measure: Effect of fructose or glucose on blood glucose Time: Baseline, and up to 3-hours after solutionsDescription: Determine the triglycerides levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose.
Measure: Effect of fructose or glucose on triglycerides levels Time: Baseline, and up to 3-hours after solutionsDescription: Determine the uric acid, pyruvic acid, and lactate levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose.
Measure: Effect of fructose or glucose on uric acid, pyruvic acid, and lactate Time: Baseline, and up to 3-hours after solutionsDescription: Determine the malondialdehyde level before and 3-hours after ingestion of 75g of fructose or 75g of glucose.
Measure: Effect of fructose or glucose on oxidative stress Time: Baseline, and up to 3-hours after solutionsDescription: TAS1R2 (taste receptor, type 1, member 2 T1R2) is the component specific to sweet taste perception, and does not change during the life. We will determine whether Ser9Cys (dbSNP: rs9701796) and Ile191Val (dbSNP: rs35874116)variations in TAS1R2 were associated with differences the sweet taste perceptions.
Measure: Effect of two polymorphisms in the gene TAS1R2 in the sweet taste perception Time: only at baselineDescription: Determine the peak plasma blood glucose before and 3-hours after ingestion of 75g of fructose or 75g of glucose. We also test capillary blood glucose before and 30, 90, 120 and 180 minutes after solutions.
Measure: Effect of fructose or glucose on blood glucose Time: Baseline, and up to 3-hours after solutionsDescription: Two different visual analogue scales will be used to assess hunger, satiety, fullness, prospective food consumption, palatability, taste, aftertaste, smell, and visual appeal of the solutions. The scales will be performed at 7, 35, 95 and 120 minutes after solutions.
Measure: Effect of fructose or glucose on appetite and palatability Time: Baseline, and up 120 minutesDescription: Determine the glucagon levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose
Measure: Effect of fructose or glucose on glucagon levels Time: Baseline, and up to 3-hours after solutionsDescription: Determine the leptin levels before and 3-hours after ingestion of 75g of fructose or 75g of glucose
Measure: Effect of fructose or glucose on leptin levels Time: Baseline, and up to 3-hours after solutions