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Report for Clinical Trial NCT02523326

Developed by Shray Alag, 2020.
SNP Clinical Trial Gene

Design of a Protocol for Obtaining Genomic DNA From Saliva Using Mouthwash: Samples Taken From Patients With Periodontal Disease

Objective The aim of this study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease. Materials and methods Saliva samples were taken from 60 patients, then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.

NCT02523326 Periodontal Disease Periodontal Disease " Periodontal Disease "Unrecognized Condition Periodontal Disease "Unrecognized Condition"
MeSH:Periodontal Diseases

1 Interventions

Name: saliva samples obtained with mouthwash

Description: saliva samples obtained with mouthwash
Type: Device
Group Labels: 3

Group A Group B Group C

Primary Outcomes

Description: high quality genomic DNA from saliva samples obtained with mouthwash.

Measure: DNA

Time: 10 days [group A], 20 days [group B], 30 days [group C]

Time Perspective: Prospective

There is one SNP


1 rs679620

To verify the quality and species (human) of the DNA extracted, primers were designed for the rs679620 polymorphism (forward 5´- ggg gCT TAA ggC ACA TgA gT-3; reverse 5´- ACT TCg ggA TgC CAg gAA Ag - 3´; Tm: 56°C, 40 cycles) that amplified a product of 485 bp.

HPO Nodes