Interactions between genes and environment, are likely to be crucial in the development of the common diseases such as type 2 diabetes. Recently, the investigators have obtained data that genotypes of genes encoding for fatty acid desaturases 1 and 2 (FADS1 and FADS2) are the strongest genes in a genome-wide analysis regulating serum fatty acid profile.The aim of this study is to test if subjects with different genotypes of the FADS2 gene respond differently to a diet supplemented with linoleic acid or alpha-linolenic acid (substrates for FADS2). The study hypothesizes that subjects will be more sensitive to the dietary modifications according to their genotype leading to more robust differences in serum FA profile, tissue inflammation and serum lipids.
Description: Fatty acid composition of serum lipid fractionsMeasure: Change in fatty acid composition of serum lipid fractions Time: 8 weeks
Description: Oral glucose tolerance test (OGTT) variables: fasting, 30 min and 120 min glucose and insulin measurementsMeasure: Glucose and insulin metabolism response Time: 8 weeks
Description: Serum circulating levels of high-sensitivity C-reactive proteinMeasure: Inflammatory markers response Time: 8 weeks
Description: Subcutaneous adipose tissue taken by open biopsy to collect 1-5 g of adipose tissue.Measure: Adipose tissue biopsies Time: 8 weeks
There is one SNP
Subcutaneous adipose tissue taken by open biopsy to collect 1-5 g of adipose tissue.. Inclusion Criteria: - Participants of METSIM study cohort with CC/TT FADS1 rs174547 SNP genotype.
Inclusion Criteria: - Participants of METSIM study cohort with CC/TT FADS1 rs174547 SNP genotype.