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Report for Clinical Trial NCT03025347
Developed by Shray Alag, 2020.
SNP Clinical Trial Gene
Effect of Exercise on Appetite, Energy Intake, Butyrylcholinesterase Activity and Gut Peptides in Men With Variants of the Obesity-linked FTO rs9939609 Polymorphism
Using a database of individuals with FTO genetic data, the study aims to assess the appetite, energy intake, butyrylcholinesterase, gut hormone responses to a bout of moderate- to high intensity exercise in individuals with genetic variations in the FTO gene.
NCT03025347 Appetitive Behavior
2 Interventions
Name: Control
Name: Exercise
Primary Outcomes
Description: Measured using ELISA from venous blood samples
Measure: Plasma acylated ghrelin concentrations (N=24) Time: 24 hoursDescription: Measured using ELISA from venous blood samples
Measure: Plasma desacyl ghrelin concentrations (N=24) Time: 24 hoursDescription: Measured using visual analogue scales
Measure: Subjective appetite (N=24) Time: 24 hoursDescription: Measured at laboratory-based meals
Measure: Ad-libitum energy intake (N=24) Time: 24 hoursDescription: Measured using Ellman's reagent protocol
Measure: Plasma butyrylcholinesterase activity (N=24) Time: First hour of main trial (three samples).Secondary Outcomes
Description: Measured using ELISA from venous blood samples
Measure: Plasma total glucagon-like peptide 1 concentrations (N=24) Time: 24 hoursDescription: Measured using ELISA from venous blood samples
Measure: Plasma total peptide yy concentrations (N=24) Time: 24 hoursDescription: Measured using ELISA from venous blood samples
Measure: Plasma leptin concentrations (N=24) Time: Baseline (fasting) samplePurpose: Basic Science
Allocation: Randomized
Crossover Assignment
There is one SNP
SNPs
1 rs9939609
Effect of Exercise on Appetite, Energy Intake, Butyrylcholinesterase Activity and Gut Peptides in Men With Variants of the Obesity-linked FTO rs9939609 Polymorphism.
Inclusion Criteria: - non-smoker, not currently dieting, weight stable for >3 months (self-reported), no personal history of cardiovascular disease, metabolic disease or dyslipidaemia, European ancestry, no psychiatric or medical condition Exclusion Criteria: - food allergies, dislike or intolerance of study foods and drinks, irregular eating patterns, use of medication that could influence hormone concentrations Inclusion Criteria: - non-smoker, not currently dieting, weight stable for >3 months (self-reported), no personal history of cardiovascular disease, metabolic disease or dyslipidaemia, European ancestry, no psychiatric or medical condition Exclusion Criteria: - food allergies, dislike or intolerance of study foods and drinks, irregular eating patterns, use of medication that could influence hormone concentrations Appetitive Behavior The fat mass and obesity-associated gene (FTO) rs9939609 A allele is related to obesity, greater food intake and impaired postprandial reduction of ghrelin.
Exercise acutely suppresses levels of ghrelin and appetite, yet whether the response differs in people with or without the rs9939609 A allele is unknown.
This study assessed the effect of exercise on appetite, appetite-regulatory hormones and energy intake in variants of the FTO rs9939609 polymorphism.
Cohort The investigators initially recruited 202 subjects to a database and measured FTO rs9939609 genotype.
From these subjects, 12 individuals homozygous for the 'obesity-risk' rs9939609 A allele and 12 homozygous for the T allele were recruited.