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Report for Clinical Trial NCT02640976

Developed by Shray Alag, 2020.
SNP Clinical Trial Gene

Assessing the Relation Between Hormone Receptors Gene Polymorphism and Ovarian Stimulation Response in In Vitro Fertilization (IVF) Program

The aim of this study is to assess the role of AMH in prediction of poor ovarian response as well as the relation between ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs and the poor response in Egyptian women undergoing IVF procedure. Discovering the genetic variants associated with ovarian response is an important step towards individualized pharmacogenetic protocols of ovarian stimulation.

NCT02640976 Infertility
MeSH:Infertility
HPO:Infertility

3 Interventions

Name: Human menopausal gonadotrophin (HMG)

Description: [Merional® 75 IU ampoules, IBSA institut]
Type: Drug
Group Labels: 2

Good responders Poor responders

Name: GnRH antagonist Cetrorelix

Description: [Cetrotide® 0.25 mg syringes, Merck Serono]
Type: Drug
Group Labels: 2

Good responders Poor responders

Name: Human chorionic gonadotrophin (hCG)

Description: [Choriomon® 5000 IU ampoules, IBSA institut]
Type: Drug
Group Labels: 2

Good responders Poor responders


Primary Outcomes

Description: when at least 3 follicles will reach 17 mm in diameter, ovulation will be triggered by a single intra-muscular injection of 10,000 IU of hCG [Choriomon® 5000 IU ampoules, IBSA institut]. 36 hours later, oocyte retrieval will be performed and will be guided by transvaginal ultrasound.

Measure: number of oocytes collected

Time: 3 weeks from of start of ICSI cycle

Secondary Outcomes

Description: By using PCR, we will indicate the genotype in each individual and determine the probability of the poor ovarian response.

Measure: Detection of the single nucleotide polymorphisms ESR2(+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR)

Time: Within 1 week

Other Outcomes

Description: Based on FSH level, the dose of HMG ( Merional) will be adjusted for each individual.

Measure: Laboratory analysis of basal follicle stimulating hormone level for each individual will be measured by enzyme-linked immunosorbent assay (ELISA)

Time: Done within 3 months preceeding the ICSI cycle

Description: Based on AMH level, the dose of HMG (Merional) will be adjusted for each individual.

Measure: Laboratory analysis of anti-mullerian hormone level (AMH) will be measured by ELISA technique for each individual.

Time: Done within 3 months preceeding the ICSI cycle.

Purpose: Screening

Allocation: Non-Randomized

Parallel Assignment


There are 3 SNPs

SNPs


1 rs4986938

Poor Ovarian Stimulation Response in In Vitro Fertilization (IVF) Program The aim of this study is to assess the role of AMH in prediction of poor ovarian response as well as the relation between ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs and the poor response in Egyptian women undergoing IVF procedure.

36 hours later, oocyte retrieval will be performed and will be guided by transvaginal ultrasound.. Detection of the single nucleotide polymorphisms ESR2(+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).

Detection of the polymorphisms ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).


2 rs6165

Poor Ovarian Stimulation Response in In Vitro Fertilization (IVF) Program The aim of this study is to assess the role of AMH in prediction of poor ovarian response as well as the relation between ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs and the poor response in Egyptian women undergoing IVF procedure.

36 hours later, oocyte retrieval will be performed and will be guided by transvaginal ultrasound.. Detection of the single nucleotide polymorphisms ESR2(+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).

Detection of the polymorphisms ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).


3 rs6166

Poor Ovarian Stimulation Response in In Vitro Fertilization (IVF) Program The aim of this study is to assess the role of AMH in prediction of poor ovarian response as well as the relation between ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs and the poor response in Egyptian women undergoing IVF procedure.

36 hours later, oocyte retrieval will be performed and will be guided by transvaginal ultrasound.. Detection of the single nucleotide polymorphisms ESR2(+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).

Detection of the polymorphisms ESR2 (+1730G>A) (rs4986938), FSHR p.Thr307Ala (c.919A>G, rs6165) and FSHR p.Asn680Ser (c.2039A>G, rs6166) SNPs will be performed using the TaqMan system by real-time polymerase chain reaction (PCR).



HPO Nodes


HPO: